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采用一阶导数光谱法测定氯地滴耳液中氯霉素的含量，测定波长为３０３．０ｎｍ。氯霉素在０～６５．２μｇｍｌ－１范围内的线性关系良好，平均回收率为９９．８４％，ＲＳＤ为０．３７％（ｎ＝８）。本法操作简单快速，方法准确可靠。
Determination of chloramphenicol in the ear drops of Rhodiola by first derivative spectrometry was performed at a wavelength of 303.0 nm. The linearity of chloramphenicol in the range of 0 ~ 65.2μgml-1 was good with an average recovery of 99.84% and RSD of 0.37% (n = 8). The method is simple and fast, the method is accurate and reliable.