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用多例提纯的ＢＪ－λ混合免疫ＢＡＬＢ／Ｃ小鼠的脾细胞与骨髓瘤细胞Ｓｐ２／０融合，获得３株稳定分泌抗人屹游离λ链ＭｃＡｂ的杂交瘤细胞株（ＡＨλＤ８、ＡＨλ１Ｃ１和ＡＨλ２Ｃ１）．它们分泌的ＭｃＡｂ均能与我室所有１７例ＢＪ－λ起强反应，但不与结合的λ型轻链及Ｋ型轻链反应，表明这３株ＭｃＡｂ可能是抗人Ｉｇ游离λ链共同抗原决定簇的．３株ＭｃＡｂ均属小鼠ＩｇＧ１亚类。添加ＥＬＩＳＡ表明它们识别相同的表位。用于检测正常人血清和尿中的游离λ链及多发性骨髓瘤患者的ＢＪ－λ均具有很好的特异性和敏感性．本文还讨论了有关的免疫问题．
The spleen cells of BALB / C mice immunized with multiple purified BJ-λ were fused with myeloma cells Sp2 / 0 to obtain three hybridoma cell lines (AHλD8, AHλ1C1 and AHλ2C1 ). McAb secreted by them all reacted strongly with all 17 BJ-λ in our laboratory, but did not react with the combined λ-type light chain and K-type light chain, indicating that the three McAbs may be anti-human Ig free λ chain common antigen Decided Three McAbs belong to mouse IgG1 subclass. ELISA was added to show that they recognize the same epitope. The BJ-λ used to detect free λ chain and multiple myeloma in normal human serum and urine has good specificity and sensitivity. This article also discusses the related immunological issues.