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目的探讨蜘蛛香环烯醚萜治疗肠易激综合征(IBS)作用机理。方法雄性SD大鼠89只,分为空白组14只,模型组、氟西汀组和蜘蛛香环烯醚萜大(24.92 mg/kg)、中(12.46 mg/kg)、小(6.23 mg/kg)3个剂量组各15只。以慢性应激加孤养的方法造模,观察蜘蛛香环烯醚萜对腹部回撤反射(AWR)压力阈值和结肠P物质(SP)、血管活性肠肽(VIP)含量的影响。结果模型组大鼠疼痛压力阈值和最大耐受压力阈值均显著降低(P<0.01),蜘蛛香环烯醚萜3个剂量组AWR值都有显著改善,尤其是大、中剂量组效果极显著(P<0.001)。模型组结肠中SP有升高趋势,VIP有降低趋势,蜘蛛香环烯醚萜各剂量组均可降低SP,以大剂量组更为明显(P<0.01),并有升高VIP和降低结肠中SP/VIP值的趋势。结论蜘蛛香环烯醚萜对胃肠道功能亢进有明显抑制作用,可降低内脏敏感性,提示蜘蛛香环烯醚萜对肠易激综合征(IBS)的症状有改善作用,机制可能与可调节胃肠激素VIP、SP有关。
Objective To investigate the mechanism of spilantol iridoid treatment of irritable bowel syndrome (IBS). METHODS: A total of 89 male Sprague-Dawley rats were randomly divided into blank control group (n = 14), model group, fluoxetine group and spironolactone large (24.92 mg / kg), middle (12.46 mg / kg) three dose groups of 15 each. The effects of arachidon iridoid on pressure drop threshold (AWR) and content of substance P (SP) and vasoactive intestinal peptide (VIP) in the colon were observed by chronic stress and solitary method. Results The pain threshold and maximal pressure threshold were significantly decreased in the model group (P <0.01). The AWR values of the three doses of spironolactone were significantly improved, especially in the large and middle dose groups (P <0.001). In the model group, the SP in the colon increased and the VIP decreased. Each dose of spironolactone decreased SP, especially in the high dose group (P <0.01), and elevated VIP and decreased colon In the SP / VIP value trend. Conclusion Spironolactone can significantly inhibit visceral hypersensitivity and reduce visceral sensitivity, suggesting that spironolactone may improve the symptoms of irritable bowel syndrome (IBS). The mechanism may be related to the Regulate gastrointestinal hormone VIP, SP related.