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采用高速枪弹射击猪单后肢软组织,观察伤后远离原发伤道的血管内皮细胞的形态学变化,为远达效应的血流扰动学说提供实验资料。动物伤后6h解剖,大体见双肺下叶、左室后壁以及大脑的背、腹侧面均有不同程度的血管破裂、出血,或毛细血管充血。光镜见猪主动脉弓内皮细胞(VEC)有水肿,或脱落呈“鞋钉样”垂直悬吊在血管腔内;连续切片见内皮层有剥脱现象。透射电镜观察,细胞间连接分离,细胞膜有水肿、或破裂;胞浆疏松,胞浆内空泡增多,细胞器数目减少。提示,高能创伤后,远隔部位的血管内皮细胞可发生明显的形态学变化,这是远达效应的基本特征。究其原因可能与致伤瞬间的剧烈流体扰动和继发性神经体液因子的变化有关。
High-speed bullets were used to shoot soft tissue of hind limbs of pigs. Morphological changes of vascular endothelial cells far away from primary wounds were observed and experimental data were provided for the teleconnection theory. Animal anatomy at 6h after injury, generally see the lower lungs, left ventricular posterior wall and the brain’s dorsal, ventral surface have varying degrees of vascular rupture, hemorrhage, or capillary congestion. Light microscopy porcine aortic arch endothelial cells (VEC) with edema, or shedding was “spike-like” vertical suspension in the vascular cavity; serial sections of the endothelium stripping phenomenon. Transmission electron microscope observation, separation of cell junctions, cell membrane edema, or rupture; loose cytoplasm, cytoplasmic vacuoles increased, reducing the number of organelles. Tip, high-energy trauma, distant parts of the vascular endothelial cells can be significant morphological changes, which is the basic characteristics of the telecontrol effect. The reason may be the moment of acute injury fluid turbulence and secondary changes in neurohumoral factors.