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目的探讨CCN家族成员的Cyr61、CTGF和Nov基因在孤立性大肝癌、结节性肝癌、小肝癌中的表达差异及其临床意义。方法比较孤立性大肝癌、结节性肝癌、小肝癌的临床病理特征,采用半定量的RT-PCR方法分析各组肝癌组织中Cyr61、CTGF和Nov基因的表达,并分析其表达临床病理特征的关系。结果83.3%(10/12)的结节性肝癌有静脉浸润,明显高于孤立性大肝癌和小肝癌(P<0.05),结节性肝癌的分化程序和肝硬化程度也明显较孤立性大肝癌和小肝癌差(P<0.05),孤立性大肝癌和小肝癌间的病理指标比较无显著性差异(P>0.05)。孤立性大肝癌中Cyr61和CTGF基因表达量均较结节性肝癌低,差异有显著性意义(P<0.01)。结论孤立性大肝癌的临床病理特征较好,且Cyr61、CTGF基因在孤立性大肝癌中的表达明显低于结节性肝癌,提示孤立性大肝癌有相对较好的肿瘤生物学行为。
Objective To investigate the differences of Cyr61, CTGF and Nov genes in CCN family members between solitary large hepatocellular carcinoma, nodular hepatocellular carcinoma and small hepatocellular carcinoma and their clinical significance. Methods The clinicopathological characteristics of isolated large hepatocellular carcinoma, nodular hepatocellular carcinoma and small hepatocellular carcinoma were compared. The expressions of Cyr61, CTGF and Nov genes in each group were analyzed by semi-quantitative RT-PCR, and their clinical and pathological features were analyzed relationship. Results 83.3% (10/12) of nodular HCC had venous infiltration, which was significantly higher than those of isolated large hepatocarcinoma and small hepatocellular carcinoma (P <0.05). The differentiation and cirrhosis of nodular HCC were also significantly more isolated Liver cancer and small liver cancer were poor (P <0.05), isolated large hepatocellular carcinoma and small hepatocellular carcinoma pathological indicators were no significant difference (P> 0.05). The expression of Cyr61 and CTGF in isolated large hepatocellular carcinoma was significantly lower than that in nodular hepatocellular carcinoma (P <0.01). Conclusion The clinical features of isolated large hepatocellular carcinoma are good, and the expression of Cyr61 and CTGF in isolated large hepatocellular carcinoma is significantly lower than that of nodular hepatocellular carcinoma, suggesting that the isolated large hepatocarcinoma has relatively good tumor biological behavior.