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目的:观察干蟾皮醇提物抗肺癌细胞生长和促凋亡的作用。方法:将A549、NCI-H460肺癌细胞悬液分别接种于96孔培养板中,细胞贴壁后依次加入不同浓度的含干蟾皮醇提物培养液干预,并设RPMI-1640培养液空白对照组。实验结束,用MTT法测定药物对细胞增殖的影响,Hoechst荧光染色法检测对细胞凋亡的影响。结果:与空白对照组比较,干蟾皮醇提物在不同浓度下对A549和NCI-H460细胞的生长均有抑制作用(P<0.05~0.001),且呈浓度依赖性;对两种细胞的半数抑制浓度(IC50)分别为15μg/ml和1.5μg/ml。不同浓度的干蟾皮醇提物作用于A549、NCI-H460细胞后,随着药物浓度梯度的增加,凋亡细胞数目明显增加,伴随出现细胞的坏死,细胞轮廓不清,甚至细胞解体。结论:干蟾皮醇提物可显著降低A549和NCI-H460细胞的活性及促进其凋亡,且呈剂量依赖性。
Objective: To observe the effect of alcohol extracts of dried toad skin on the growth and apoptosis of lung cancer cells. Methods: A549 and NCI-H460 lung cancer cell suspension were inoculated into 96-well plates respectively. After adherent cells were treated with different concentrations of alcohol extract of dried toad skin, the cells were incubated with RPMI-1640 medium group. At the end of the experiment, the effect of drugs on cell proliferation was determined by MTT assay and the effect of Hoechst fluorescence staining on apoptosis was detected. Results: Compared with the blank control group, the ethanol extract of dried toad skin inhibited the growth of A549 and NCI-H460 cells at different concentrations (P <0.05 ~ 0.001) in a concentration-dependent manner. The median inhibitory concentration (IC50) was 15 μg / ml and 1.5 μg / ml, respectively. After the different concentrations of alcohol extract of dried toad skin were treated with A549 and NCI-H460 cells, the number of apoptotic cells increased obviously with the increase of drug concentration gradient. With the necrosis of cells, the cell outline was unclear, and even the cells disintegrated. Conclusion: The extract of dried toad skin can significantly reduce the activity and promote the apoptosis of A549 and NCI-H460 cells in a dose-dependent manner.