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目的建立实验性大鼠Walker-256肝微小转移癌模型并探讨其病理学和影像学特点。方法SD大鼠30只经脾脏注入Walker-256瘤细胞建立大鼠微小肝转移癌模型。在接种瘤细胞第9天行CT检查观察肿瘤生长情况。病理学用HE染色和血管内皮生长因子(VEGF)、微血管密度(MVD)免疫组织化学的方法观察肿瘤的大小、形态及血管化程度。结果SD大鼠30只中有2只麻醉意外死亡,剩余大鼠中随机选取22只处死,有19只肝脏内找到转移灶。转移灶的直径在0.5~6.6mm。转移的成功率为86.4%。CT常规扫描方法对直径小于5mm的转移灶检出率不足10%。病理学病灶符合癌肉瘤的特点。肿瘤内均有VEGF的表达,但程度不同;MVD的数量与VEGF表达程度呈正相关。结论经脾脏注入Walker-256癌肉瘤细胞建立大鼠肝转移癌模型的方法简便易行,成瘤率高。免疫组织化学方法显示微小转移癌灶内有新血管生成,适合影像学功能成像技术与肿瘤血管化程度的对照研究。
Objective To establish a rat model of Walker-256 hepatic micrometastasis and to explore its pathological and imaging features. Methods Thirty Sprague-Dawley rats were injected with Walker-256 tumor cells via spleen to establish a model of small liver metastasis in rats. On day 9 of inoculation of tumor cells, CT examination was performed to observe tumor growth. Pathology HE staining and vascular endothelial growth factor (VEGF), microvessel density (MVD) immunohistochemistry method to observe the size of the tumor, morphology and degree of vascularization. Results Two of the 30 SD rats died of anesthesia. Twenty-two of the remaining rats were randomly selected and sacrificed. Twenty-nine of them were found to have metastases. The diameter of metastasis is 0.5 ~ 6.6mm. The success rate of transfer was 86.4%. CT conventional scanning method less than 5mm diameter metastases detection rate of less than 10%. Pathological lesions consistent with the characteristics of carcinosarcoma. The expression of VEGF in tumors both had different degrees, but the number of MVD was positively correlated with the expression of VEGF. Conclusion The method of establishing rat model of hepatic metastasis by injecting Walker-256 carcinoma cells into the spleen is simple and easy, and has a high rate of tumorigenesis. Immunohistochemistry showed that there was neovascularization in the micrometastasis foci, which was suitable for the comparative study of imaging functional imaging and tumor vascularization.