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目的:研究弗氏葡糖杆菌产核糖醇脱氢酶的培养条件。方法:采用单因素实验研究了葡萄糖、氮源、金属离子Zn2+和Fe3+以及诱导物核糖醇对弗氏葡糖杆菌产酶的影响,并用优化的条件培养产酶进行核糖醇生物转化反应。结果:对于弗氏葡糖杆菌核糖醇脱氢酶的生产,当葡萄糖浓度2.5%、氮源玉米浆粉为1.0%、Zn2+浓度为4μmol/L、Fe3+浓度为2μmol/L时,对应的核糖醇脱氢酶活力分别为0.45U/mg、0.43U/mg、0.25U/mg和0.18 U/mg,生物转化验证实验表明反应30 h后核糖醇的转化率达97.73%,质谱分析表明产品符合L-核酮糖的结构。结论:实验获得的优化条件可进一步用于指导生产。
Objective: To study the culture conditions of ribose dehydrogenase produced by Gluconobacter spp. Methods: The effects of glucose, nitrogen source, metal ions Zn2 + and Fe3 +, and inducer ribitol on glucoaminergic glucoamylase production were investigated by single factor experiments. The biotransformation reaction of glucitol was carried out with the optimized conditions. Results: For Gluconobacter freundii ribose dehydrogenase production, when the glucose concentration was 2.5%, nitrogen source corn steep powder was 1.0%, Zn2 + concentration was 4μmol / L and Fe3 + concentration was 2μmol / L, the corresponding ribitol Dehydrogenase activity was 0.45U / mg, 0.43U / mg, 0.25U / mg and 0.18U / mg, respectively. The results of bioconversion experiments showed that the conversion of ribitol reached 97.73% after 30 h reaction. The structure of ribulose. Conclusion: The experimental optimization conditions can be further used to guide the production.