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目的评估兔成视网膜细胞瘤(retinoblastoma,RB)模型的建立及经导管眼动脉灌注(selective ophthalmic arterial injection,SOAI)治疗兔RB的可行性。方法 HXO-Rb44RB细胞予中心细胞室培养,取对数生长期HXO-Rb44细胞,用PBS配成浓度为2.0×107个/ml的细胞悬液,抽取0.1 ml细胞悬液注射于6只SPF级新西兰兔右眼视网膜间隙下。接种肿瘤细胞前3 d和注射后1个月臀部注射环孢素A 15 mg·kg-1·d-1,1个月后减量至10 mg·kg-1·d-1并持续到实验结束。将RB兔分为对照组3只和实验组3只,对照组灌注0.9%氯化钠溶液15 ml,实验组将美法仑2.5 mg用0.9%氯化钠溶液稀释至15 ml后灌注。术后分别于7、14 d比较两组肿瘤生长情况。结果注射环孢素A期间,实验兔不良反应轻微,6只均建模成功。7次插管中6次成功插至眼动脉。SOAI术后1周,实验组肿瘤可见明显缩小,2周后均消失。SOAI术后,实验组仅1只见右眼轻度红肿、流泪,1周后红肿自行消退。结论 SOAI治疗RB兔具有可行性,为进一步经眼动脉灌注治疗RB的基础研究提供了参考。
Objective To evaluate the establishment of RB model and the feasibility of treating RB with selective ophthalmic arterial injection (SOAI). Methods HXO-Rb44RB cells were cultured in the central cell culture chamber. HXO-Rb44 cells in logarithmic growth phase were collected and mixed with PBS to make a cell suspension with a concentration of 2.0 × 107 cells / ml. 0.1 ml cell suspension was injected into 6 SPF New Zealand rabbit under the right eye retinal space. Cyclosporine A (n = 15) was injected into the buttocks 3 days before inoculation and 1 month after injection, and then was reduced to 10 mg · kg-1 · d-1 End. RB rabbits were divided into control group 3 and experimental group 3, the control group perfusion of 0.9% sodium chloride solution 15 ml, the experimental group will be melphalan 2.5 mg 0.9% sodium chloride solution diluted to 15 ml perfusion. The tumor growth was compared between the two groups on day 7 and day 14 postoperatively. Results During the injection of cyclosporine A, the rabbits had mild adverse reactions and all 6 models were successfully established. Intubation in 7 times 6 times successfully inserted into the ophthalmic artery. One week after SOAI, the tumors in the experimental group were obviously reduced, and disappeared after two weeks. After SOAI, the experimental group only one see the right eye mild redness, tearing, red swelling after 1 week subsided. Conclusion The SOAI treatment of RB rabbits is feasible, which provides a reference for the further study of RB based on intra-arterial infusion.