目的探讨宫内缺氧对40日龄幼年大鼠齿状回神经干细胞(NSCs)增殖的影响及当归对NSCs的干预作用。方法实验室配种得孕SD大鼠(39只),喂养至孕14d,随机分为对照组(12只)、缺氧组(12只)和当归组(15只)。将缺氧组大鼠置于三气培养箱内缺氧[氧体积分数为130mL/L,温度25℃,相对湿度70%～75%,二氧化碳(CO2)体积分数为0.3～0.4mL/L],缺氧2h/d,连续缺氧3d(孕第14、15、16天),制作宫内缺氧脑损伤模型,缺氧组缺氧前1h经尾静脉注射9g/L盐水(8mL/kg)。当归组缺氧前1h经尾静脉注射当归注射液(8mL/kg),其余干预方法同缺氧组。对照组经尾静脉注射9g/L盐水,不缺氧。自然生产,喂养新生大鼠至产后第40天,每组随机取幼年大鼠脑组织:对照组12只、缺氧组12只、当归组15只;常规石蜡切片后,行巢蛋白(Nestin)免疫组织化学染色,在显微镜(×200)下观察Nestin阳性物质的表达,组间比较采用单因素方差分析。结果缺氧组Nestin阳性染色面积比对照组大,差异有统计学意义(P<0.05),当归组Nestin阳性染色面积比缺氧组小,差异有统计学意义(P<0.05),当归组面积较对照组增大,但差异无显著意义(P>0.05);3组平均吸光度、积分吸光度两两比较,差异均无显著意义。结论氧体积分数130mL/L的缺氧环境促进了幼年大鼠齿状回NSCs的增殖,当归注射液可减轻宫内缺氧后幼年大鼠NSCs的增殖反应。 Objective To investigate the effects of intrauterine hypoxia on the proliferation of neural stem cells (NSCs) in dentate gyrus of 40-day-old juvenile rats and the intervention of angelica on NSCs. Methods The pregnant SD rats (39) were bred in the laboratory and fed to the 14th day of pregnancy. They were randomly divided into control group (12), hypoxia group (12) and angelica group (15). Hypoxia rats were placed in a three-gas incubator for hypoxia [oxygen volume was 130 mL/L, temperature was 25°C, relative humidity was 70% to 75%, and carbon dioxide (CO2) volume fraction was 0.3 to 0.4 mL/L] , Hypoxia 2h/d, Continuous hypoxia 3d (pregnancy 14, 15 and 16 days), Intrauterine hypoxic brain injury model was established, and the hypoxic group was injected with 9 g/L saline via tail vein 1 h before hypoxia (8 mL/kg). ). Angelica injection (8 mL/kg) was injected into the tail vein 1 h before the hypoxia in the angelica group. The rest of the interventions were in the hypoxic group. The control group was injected with 9 g/L saline via the tail vein without hypoxia. Natural production, feeding neonatal rats to the 40th day after childbirth, each group was randomly taken from the brain tissue of young rats: 12 in the control group, 12 in the hypoxia group, 15 in the angelica group; nestin was performed after routine paraffin sections. Immunohistochemical staining was performed to observe the expression of Nestin-positive substances under a microscope (×200). One-way analysis of variance was used for comparison between groups. Results The area of ​​positive staining for Nestin in hypoxic group was larger than that in control group (P<0.05). The area of ​​Nestin positive staining in Angelica group was smaller than that in hypoxic group (P<0.05). The area of ​​Angelica group was significant. Compared with the control group, there was no significant difference (P>0.05). The average absorbance and integral absorbance of the three groups were not significantly different. Conclusion The hypoxic environment with an oxygen concentration of 130 mL/L promotes the proliferation of NSCs in the dentate gyrus of juvenile rats. Angelica injection can reduce the proliferative response of NSCs in young rats after intrauterine hypoxia.