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目的:建立超高效液相色谱-串联质谱(UPLC-MS/MS)法测定血浆中伏立康唑血药浓度方法。方法:以卡马西平为内标,用蛋白沉淀前处理方法;采用Acquity UPLC BEH C_(18)(100 mm×2.1 mm,1.7μm)色谱柱,流动相为乙腈-0.1%甲酸水溶液(35∶65),流速为0.4 mL/min和正离子模式多反应监测(MRM)扫描分析,并考察其专属性、标准曲线与定量下限、精密度与回收率、基质效应和稳定性。结果:血浆中伏立康唑血药浓度的线性范围为0.1~20.0μg/mL(r=0.997 0),定量下限为0.1μg/mL,提取回收率为93.22%~95.43%之间,日内、日间精密度相对标准偏差(RSD)均小于15%。结论:该法操作简便快速,特异性强,灵敏度高,可用于伏立康唑的血药浓度监测。
Objective: To establish a method for the determination of voriconazole in plasma by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS). Methods: Carbamazepine was used as internal standard and pretreated by protein precipitation. The mobile phase consisted of acetonitrile-0.1% formic acid aqueous solution (35: 1) with the mobile phase of Acquity UPLC BEH C 18 (100 mm × 2.1 mm, 65), a flow rate of 0.4 mL / min, and positive ion mode MRM scans were performed and their specificity, standard curves and quantitation limits, precision and recovery, matrix effects and stability were examined. Results: The linear range of plasma voriconazole plasma concentration was 0.1 ~ 20.0μg / mL (r = 0.997 0), the lower limit of quantitation was 0.1μg / mL and the recovery was 93.22% ~ 95.43%. The intra- and inter-day precision The relative standard deviations (RSDs) were less than 15%. Conclusion: The method is simple, rapid, specific and sensitive and can be used for the monitoring of voriconazole in plasma.