论文部分内容阅读
1988年10月～1990年10月,我们对山东部分地区进行了恙虫病血清流行病学调查。人血清标本分别采自济南、临沂和泰安等地健康人,发热待查和可疑恙虫病住院患者,同时搜集临床和流行病学资料,并从调查点捕鼠,用滤纸条采血,室温干燥,-20℃保存备检。人血清和鼠血清抗恙虫病立克次体IgG 和IgM 分别采用斑点酶染色法和免疫荧光法检测。恙虫病抗原片、抗人IgG 和IgM 酶结合物、抗鼠IgG 荧光抗体及阳性对照血清均由军
From October 1988 to October 1990, we conducted a serological epidemiological investigation of tsutsugamushi in parts of Shandong. Human serum samples were collected from healthy people in Jinan, Linyi and Tai’an, fever patients and suspected scrub typhus inpatients, clinical and epidemiological data collected at the same time, and from the investigation point mouse, using filter paper blood, dry at room temperature , -20 ℃ save for inspection. Rickettsia IgG and IgM of human serum and murine serum against scrub typhus were detected by dot blot and immunofluorescence respectively. Scrub typhus antigen tablets, anti-human IgG and IgM enzyme conjugates, anti-mouse IgG fluorescent antibody and positive control serum by the military