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合成了一种新的含磷有机三足体衍生物N-二(苯-二氨基甲酰基)甲基磷酸及其铕(Ⅲ)配合物,通过元素分析、红外光谱、差热-热重等方法对其进行结构表征和组成分析,并以该配合物为杂交探针,对其电化学传感器进行较为系统的研究。结果表明:此配体与Eu(Ⅲ)离子倾向于形成1∶1型配合物;紫外光谱法和循环伏安法的研究结果显示,铕(Ⅲ)配合物与DNA以插入形式发生相互作用。将该配合物作为杂交探针进行DNA电化学传感器研究时发现,该配合物在修饰单链DNA的电极检测作用下,无明显的电化学信号响应。而当将其用于检测杂交双链DNA时,出现了明显信号。基于该配合物的DNA传感器对互补序列、错配序列及非互补序列有良好的选择作用。
A new phosphorus-containing organic triazole derivative N-di (benzene-dicarbamoyl) methylphosphonic acid and its europium (Ⅲ) complex were synthesized and characterized by elemental analysis, IR, DTA- Methods The structure and characterization of the complexes were studied. The electrochemical probes were systematically studied by using the complexes as hybridization probes. The results show that the ligand and Eu (Ⅲ) ions tend to form a 1: 1 complex. The results of UV spectroscopy and cyclic voltammetry show that the interaction between europium (Ⅲ) complex and DNA inserted. When the complex was used as a hybridization probe for DNA electrochemical sensor, it was found that the complex had no obvious electrochemical signal response under the detection of single-stranded DNA. When it was used to detect hybridized double-stranded DNA, a clear signal appeared. DNA complexes based on this complex have good selectivity for complementary, mismatched and non-complementary sequences.