论文部分内容阅读
目的 研究细针穿刺检测涎腺肿瘤组织中细胞端粒酶活性的可行性,探讨端粒酶活性在涎腺肿瘤组织中的表达及意义。方法 采用聚合酶链式反应一端粒末端重复序列(PCR-TRAP)技术检测45例涎腺肿瘤细针穿刺标本中细胞的端粒酶。结果 33例良性肿瘤标本中3例端粒酶阳性,12例恶性肿瘤标本中10例端粒酶阳性。端粒酶活性检测与细胞学诊断符合率66.7％。二种方法同时应用可使确诊率提高至83.3％。结论涎腺肿瘤组织细针穿刺标本端粒酶检测方法具备敏感性高、特异性好的特点,与细针穿刺细胞学检测技术联合应用,在涎腺肿瘤的诊断治疗、预后方面有十分广阔的应用前景。
Objective To investigate the feasibility of detecting telomerase activity in salivary gland tumor by fine-needle aspiration and to explore the expression and significance of telomerase activity in salivary gland tumor. Methods Polymerase chain reaction-telomeric repeat repeats (PCR-TRAP) technique was used to detect the telomerase activity of 45 salivary gland fine needle aspiration specimens. Results Telomerase activity was positive in 3 of 33 benign tumors and 10 in 12 malignant tumors. The coincidence rate of telomerase activity and cytology was 66.7%. The two methods can be used simultaneously to improve the diagnosis rate of 83.3%. Conclusion The method for detecting telomerase in salivary gland tumor fine needle aspiration specimens has the characteristics of high sensitivity and good specificity. Combined with fine needle aspiration cytology, it has a very wide range in the diagnosis, treatment and prognosis of salivary gland tumors Application prospects.