论文部分内容阅读
咪唑-HCl缓冲液是现代生物化学研究中最广泛使用的缓冲液之一。本文研究了咪哩缓冲液的主要成分对Lowry法测定蛋白质的影响和对鸡胚胎脑匀浆液中蛋白质测定的影响。工作溶液牛血清白蛋白液(BSA)1mg·mL-1用逆渗透水稀释为400,200μg·mL-1。咪叹一HCl缓冲液(pH6.8,50mm
Imidazole-HCl buffers are one of the most widely used buffers in modern biochemical research. This article studies the effect of the main constituents of the micril buffer on the Lowry method for the determination of protein and the determination of proteins in chicken embryo brain homogenates. Work solution Bovine serum albumin solution (BSA) 1mg · mL-1 diluted with reverse osmosis water to 400,200μg · mL-1. Mi sigh a HCl buffer (pH6.8, 50mm