目的研究二十碳五烯酸(EPA)对人树突状细胞成熟过程中基因表达谱的影响。方法采用GM-CSF及IL-4诱导人外周单核细胞获得非成熟树突状细胞,然后将细胞分为三组处理,A组,维持树突状细胞的未成熟状态;B组,LPS(100ng/ml)作用48h诱导树突状细胞的成熟;C组,经EPA(50μmol/L)预处理24h后再予以LPS(100ng/ml)诱导树突状细胞的成熟。用低通量的cDNA表达谱芯片检测三组细胞基因表达谱的情况。RT-PCR法验证相关差异表达基因的结果。结果在LPS诱导树突状细胞成熟的过程中共有29条基因表达增加,9条基因表达下降。但是经EPA孵育24h后再予以LPS诱导“成熟”的人树突状细胞其基因表达水平发生明显变化,与未经EPA处理的成熟树突状细胞比较,共有15条基因出现差异表达,其中在29条本该上调的基因中有11条基因表达出现下降,1条基因出现上调。在9条本该表达下调的基因中有1条基因出现上调,2条明显下调。RT-PCR验证了其中6条基因的芯片结果。结论 EPA对树突状细胞的成熟过程中多种基因的表达具有明显的抑制作用,其范围涉及细胞因子及抗原提呈分子等不同基因。 Objective To investigate the effect of eicosapentaenoic acid (EPA) on the gene expression profile of human dendritic cells during maturation. Methods Human peripheral blood mononuclear cells (PBMCs) were induced by GM-CSF and IL-4 to obtain immature dendritic cells. The cells were divided into three groups to maintain the immature state of dendritic cells. Group B, LPS 100ng / ml) for 48h. Group C was pretreated with EPA (50μmol / L) for 24 hours and then induced with LPS (100ng / ml) to induce dendritic cell maturation. Low-throughput cDNA microarray was used to detect the gene expression profiles of three groups of cells. RT-PCR method to verify the results of differentially expressed genes. Results In the course of LPS-induced dendritic cell maturation, a total of 29 genes were increased and 9 genes were decreased. However, the gene expression levels of dendritic cells changed significantly after incubation with EPA for 24 h and then induced by LPS. Compared with mature dendritic cells without EPA treatment, a total of 15 genes were differentially expressed, Of the 29 genes that are up-regulated, 11 have a decreased gene expression and one has an up-regulated gene. One of the nine genes that were down-regulated had an up-regulation and two were significantly down-regulated. RT-PCR verified the chip results of six genes. Conclusion EPA can significantly inhibit the expression of many genes in the process of dendritic cell maturation, and its scope involves different genes such as cytokines and antigen presenting molecules.