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目的:观察JNK在肾缺血-再灌注损伤大鼠肾组织中的表达,研究银杏达莫注射液对肾缺血-再灌注损伤的防治作用。方法:将健康雄性SD大鼠50只随机分为5组:假手术对照组(n=10),缺血-再灌注损伤模型组(n=10),银杏达莫高剂量、中剂量、低剂量组(每组10只)。对于模型组、高、中、低剂量组,切除右肾,夹闭左侧肾蒂缺血1h,移去动脉夹再灌注1h,除此之外,对于银杏达莫处理组,手术前分别按每天0.9,1.8,3.6ml/kg的剂量尾静脉注射给药1周,假手术对照组和模型组手术前按每天1.8ml/kg的剂量尾静脉注射生理盐水,给药1周。检测肾组织丙二醛(MDA)含量、超氧化物歧化酶(SOD)的活性及血肌酐(Scr)、血尿素氮(BUN)的含量。取肾组织光镜、电镜下观察各组肾组织的形态学变化,用RT-PCR技术检测各组大鼠肾组织JNKmRNA的表达情况,Western印迹法观察p38MAPK、JNK的活化情况。结果:与假手术对照组相比,模型组大鼠Scr(214.2±40.1)μmol/L、BUN(8.75±1.28)mmol/L及MDA(11.27±2.43)nmol/mgprot含量均比假手术组增高(P<0.01),SOD(103.62±6.59)nmol/mgprot活性显著下降(P<0.01);肾组织JNKmRNA(1.38±1.36)水平显著升高(P<0.01);肾组织p-JNK蛋白水平显著上升,与模型组比较差异有统计学意义(P<0.05)。银杏达莫注射液干预后,Scr、BUN及MDA含量显著降低,SOD活性显著升高;肾组织JNKmRNA水平显著下调,与模型组比较差异有统计学意义(P<0.01);肾组织p-JNK蛋白水平显著下调,与模型组比较差异有统计学意义(P<0.05);银杏达莫不同剂量组之间差异无统计学意义。结论:银杏达莫注射液可通过降低氧自由基水平、减轻脂质过氧化反应,从而下调JNKmRNA及蛋白表达水平以改善缺血-再灌注损伤大鼠肾功能,减轻其损伤程度。
Objective: To observe the expression of JNK in renal tissue of rats with renal ischemia-reperfusion injury and to study the preventive and therapeutic effects of Yinxingdamo injection on renal ischemia-reperfusion injury. Methods: Fifty healthy male Sprague-Dawley rats were randomly divided into 5 groups: sham operation control group (n = 10), ischemia-reperfusion injury model group (n = 10) Dose group (10 in each group). For the model group, high, medium and low dose group, the right kidney was excised, the left renal pedicle was occluded ischemia for 1 hour, the arterial clip was removed and reperfusion for 1 hour. In addition, for the ginkgo dipyridamole group, 0.9, 1.8, 3.6ml / kg daily dose of tail vein injection for 1 week, sham operation control group and model group before surgery, the daily dose of 1.8ml / kg dose of intravenous injection of normal saline for 1 week. The content of malondialdehyde (MDA), superoxide dismutase (SOD) and the content of serum creatinine (Scr) and blood urea nitrogen (BUN) in renal tissue were measured. The morphological changes of renal tissues were observed under light microscope and electron microscope. The expression of JNK mRNA in renal tissues of each group was detected by RT-PCR. The activation of p38MAPK and JNK was observed by Western blotting. Results: Compared with the sham-operation group, Scr (214.2 ± 40.1) μmol / L, BUN (8.75 ± 1.28) mmol / L and MDA (11.27 ± 2.43) nmol / (P <0.01), while the activity of nmol / mgprotin (103.62 ± 6.59) decreased significantly (P <0.01); the level of JNK mRNA (1.38 ± 1.36) in renal tissue was significantly increased Rise, compared with the model group, the difference was statistically significant (P <0.05). The levels of Scr, BUN and MDA were significantly decreased and the activity of SOD was significantly increased after the intervention of Ginkgo biloba injection. The levels of JNK mRNA in kidney were significantly decreased (P <0.01), and the expression of p-JNK (P <0.05). There was no significant difference between different dosage groups of ginkgodam (P <0.05). Conclusion: Ginkgo biloba injection can reduce the level of oxygen free radicals, reduce lipid peroxidation, thereby reducing the expression of JNK mRNA and protein in rats with ischemia-reperfusion injury to improve renal function and reduce the degree of injury.