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采用多种诊断方法,对2008年以来湖北省多个地区新引进育肥肉牛呼吸道传染病进行了病原学研究。患牛病理变化主要集中在肺部,轻者可见肺局部肉样变,严重者可见肺广泛分布干酪样或化脓性坏死灶。将肺坏死组织制作石蜡切片,显微镜观察发现,肺组织表现为坏死性肺炎,未见典型的结核结节。实验室病原检测项目包括:支原体培养鉴定,PCR检测与目的基因测序鉴定;细菌分离鉴定;牛结核检测;泰勒虫血涂片检测与PCR检测等。最终确定该新发呼吸道传染病为牛支原体(Mycoplasma bovis)肺炎。同时,环形泰勒虫混合感染与细菌继发感染率很高,使病情复杂化。通过药敏试验发现,不同牛场分离的牛支原体最敏感药物为环丙沙星,其次为左氟沙星、四环素。文中还对该病的综合防治提供了合理的建议。
Using a variety of diagnostic methods, etiological studies were conducted on newly introduced respiratory fatal cattle infectious diseases in many areas of Hubei Province since 2008. Histopathological changes in cattle mainly concentrated in the lungs, the light can be seen in local lung meat samples change, severe cases of lung can be seen widely distributed caseous or pyogenic necrotic lesions. Paraffin sections were made from necrotic lung tissue. Microscopic examination showed that the lung tissue showed necrotizing pneumonia and no typical tuberculous nodules. Laboratory pathogen testing items include: Mycoplasma culture identification, PCR detection and the purpose of gene sequencing identification; bacterial isolation and identification; bovine tuberculosis detection; Tyler parasitic blood smear detection and PCR detection. The new respiratory infectious disease was eventually determined to be Mycoplasma bovis pneumonia. At the same time, the ring-shaped Tyrannus mixed infection and bacterial secondary infection rate is high, making the disease complicated. Through drug susceptibility test, it was found that the most sensitive drug of M. bovis isolated from different farms was ciprofloxacin, followed by levofloxacin and tetracycline. The article also provides reasonable advice on the comprehensive prevention and treatment of the disease.