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本文报导了人精浆的免疫抑制作用及其机制的研究.我们发现在含有10%混合的 AB~+正常人血清(NHS)同时加入 ConA 和不同浓度 HSP 的条件下培养,人外周血淋巴细胞仍存活,但淋巴细胞转化受到抑制。并且,ConA 活化的淋巴细胞表面 IL—2受体增生受抑制。加入外源性 IL—2能部分地抵消低浓度 HSP 的作用;但是,加入外源性 IL—1对 HSP 的免疫抑制作用无影响。SDS—聚丙烯酰胺凝胶电泳和放射自显影结果表明 HSP 可将~(125)Ⅰ标记的 IL—2至少降解成2个小分子片段。这些结果提示:通过影响 IL—2的产生;降解已产生的 IL—2和减少淋巴细胞表面 IL—2受体的表达可能是 HSP 抑制淋巴细胞转化和增殖的原因之一。这一抑制作用防止了针对精子免疫反应的发生。
This article reports the immunosuppressive effects of human seminal plasma and its mechanism.We found that in 10% mixed AB ~ + normal human serum (NHS) while adding ConA and different concentrations of HSP culture conditions, human peripheral blood lymphocytes Still survive, but lymphocyte transformation is inhibited. Moreover, ConA-activated lymphocyte surface IL-2 receptor proliferation is inhibited. The addition of exogenous IL-2 partially offset the effect of low concentration of HSP; however, the addition of exogenous IL-1 had no effect on the immunosuppressive effect of HSP. SDS-polyacrylamide gel electrophoresis and autoradiography showed that HSP could degrade ~ (125) Ⅰ labeled IL-2 into at least two small fragments. These results suggest that one of the reasons why HSP inhibits lymphocyte transformation and proliferation may be through the influence of IL-2 production; degradation of IL-2 production and reduction of IL-2 receptor expression on lymphocytes. This inhibition prevents the development of sperm immune responses.