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用单氨基聚乙二醇(m PEG-NH2)引发ε-三氟乙酰基-L-赖氨酸-N-羧酸酐(Lys(TFA)-NCA)开环聚合,得到了聚乙二醇-b-聚(ε-三氟乙酰基-L-赖氨酸)(PEG-b-PTLL)两嵌段共聚物.将PTLL链段末端的NH2与2-溴异丁酰溴反应得到了大分子引发剂,通过原子转移自由基聚合(ATRP)的方法分别分别引发苯乙烯(St)和N-异丙基丙烯酰胺(NIPAM)聚合,制备了结构明确、聚合度可控的聚乙二醇-b-聚(ε-三氟乙酰基-L-赖氨酸)-b-聚苯乙烯(PEG-b-PTLL-b-PS)和聚乙二醇-b-聚(ε-三氟乙酰基-L-赖氨酸)-b-聚(N-异丙基丙烯酰胺)(PEG-b-PTLL-b-PNIPAM)三嵌段杂化共聚肽.将PEG-b-PTLL-b-PNIPAM去保护后得到温度和p H响应三嵌段共聚物;将PEG-b-PTLL-b-PS去保护后得到p H响应的两亲性三嵌段共聚物.研究了PEG45-b-PLL106-b-PS20在混合溶剂H2O/DMF中的p H诱导胶束化行为.TEM结果表明,当水溶液p H小于PLL的p Ka时,PEG45-bPLL106-b-PS20形成球状胶束,当水溶液p H大于PLL的p Ka时,PLL转变成α-螺旋,PEG45-b-PLL106-b-PS20组装成盘状胶束.
The ring-opening polymerization of ε-trifluoroacetyl-L-lysine-N-carboxy anhydride (Lys (TFA) -NCA) with monoamino polyethylene glycol (m PEG-NH2) gave a polyethylene glycol- b-poly (ε-trifluoroacetyl-L-lysine) (PEG-b-PTLL) diblock copolymer The reaction of NH2 at the end of the PTLL segment with 2-bromoisobutyrylbromide gave the macromolecule Initiator and the polymerization of styrene (St) and N-isopropylacrylamide (NIPAM) were induced by atom transfer radical polymerization (ATRP) respectively to prepare a polyethylene glycol- (ε-trifluoroacetyl-L-lysine) -b-polystyrene (PEG-b-PTLL-b-PS) and polyethylene glycol-b- -L-Lysine) -b- poly (N-isopropylacrylamide) (PEG-b-PTLL-b-PNIPAM) triblock hybridized peptide PEG-b-PTLL-b-PNIPAM After the protection, the temperature and p H response triblock copolymer was obtained, and the p H -responsive amphipathic triblock copolymer was obtained after deprotection of PEG-b-PTLL-b-PS.The effects of PEG45-b-PLL106-b -PS20 in a mixed solvent H2O / DMF.The TEM results showed that the PEG45-bPLL106-b-PS20 formed spherical micelles when the pH of the aqueous solution was less than p Ka of the PLL, and when the aqueous solution p H was greater than P When the p Ka of LL was converted to α-helix, PEG45-b-PLL106-b-PS20 was assembled into discotic micelles.