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目的:探讨汉防己甲素(Tet)对人鼻咽癌细胞株CNE1和CNE2的放射增敏作用及其机制。方法:MTT法检测细胞增殖抑制作用,克隆形成实验比较细胞放射敏感性,流式细胞术检测细胞周期分布。结果:Tet对CNE1和CNE2细胞的最大非细胞毒性剂量分别为1.5μmol/L和1.8μmol/L,该浓度的Tet联合放射线照射与单纯放射线照射相比,在培养至第4～6 d能明显抑制细胞增殖(P<0.01)。CNE1和CNE2细胞单纯放射线照射组平均致死剂量(Do)分别为(1.26±0.02)Gy和(2.27±0.04)Gy,Tet联合放射线照射组Do分别为(0.73±0.05)Gy和(1.61±0.08)Gy,放射增敏比分别为1.73和1.40(P<0.05)。单纯放射线照射组CNE1和CNE2细胞周期分布以G2期为主,分别为(42.62±2.07)%和(34.82±2.74)%,Tet联合放射线照射组CNE1和CNE2 G2期比例分别为(17.02±1.87)%和(19.64±4.82)%(P<0.01)。结论:Tet能增加人鼻咽癌细胞株CNE1和CNE2对放射线照射的敏感性,机制可能与去除放射线照射诱导的G2期阻滞有关。
AIM: To investigate the radiosensitization effect of tetrandrine (Tet) on human nasopharyngeal carcinoma cell line CNE1 and CNE2 and its mechanism. Methods: MTT assay was used to detect the cell proliferation inhibitory effect. The colony formation assay was used to compare the cell radiosensitivity. The cell cycle distribution was analyzed by flow cytometry. Results: The maximum cytotoxic doses of Tet to CNE1 and CNE2 cells were 1.5 μmol / L and 1.8 μmol / L, respectively. Compared with pure radiation, the concentrations of Tet combined with radiotherapy showed significant differences Inhibition of cell proliferation (P <0.01). The average lethal dose (Do) of CNE1 and CNE2 cells in radiotherapy alone group was (1.26 ± 0.02) Gy and (2.27 ± 0.04) Gy, respectively, while that in Tet combined radiation group was (0.73 ± 0.05) Gy and (1.61 ± 0.08) Gy, radiosensitivity ratios were 1.73 and 1.40, respectively (P <0.05). The cell cycle distributions of CNE1 and CNE2 in radiotherapy group were (42.62 ± 2.07)% and (34.82 ± 2.74)%, respectively. The proportions of CNE1 and CNE2 G2 in Tet combined radiation group were (17.02 ± 1.87)% and % And (19.64 ± 4.82)% (P <0.01). Conclusion: Tet can increase the sensitivity of human nasopharyngeal carcinoma cell lines CNE1 and CNE2 to radiation. The mechanism may be related to the G2 arrest induced by radiation.