论文部分内容阅读
我们于七九年春、夏两季连续进行了油菜花药培养研究,接种材料共27份,以甘兰型(Brassica nupus)油菜品种间杂种一、二代材料为主,尚有部份远缘杂交及其它材料。接种花蕾用漂白粉液消毒,采用不带或少带花丝的花药接种,去分化阶段进行遮光培养,温度为20℃左右,再分化阶段每天补加日光灯照9—10小时,温度为18—24℃。全年共接种油菜花药10,000余个,诱导出愈伤组织4,732块,总诱导率为47.2％。转接愈伤组织1876块,分化出绿苗275苗,绿苗分化率达14.7％。在获得大批绿苗基础
In the spring and summer of 1979, we conducted a series of research on the anther culture of rapeseed. The inoculums were 27 in total. The first and second generation hybrids of Brassica napus cultivars were dominant, with some distant hybridization And other materials. Inoculation flower buds with bleach solution disinfection, with or without filament anther inoculation, to the differentiation stage shading culture, the temperature is about 20 ℃, redifferentiation stage daily add fluorescent lamp 9-10 hours, the temperature is 18-24 ℃ . More than 10,000 canola anthers were inoculated throughout the year, and 4,732 callus were induced with a total induction rate of 47.2%. Transfer 1876 callus, differentiation of green seedling 275 seedlings, green seedlings differentiation rate of 14.7%. Get a large number of green seedling foundation