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用改良胶原酶消化技术和聚蔗糖密度梯度离心方法将大鼠胰岛从胰腺分离,按Sun等的方法用海藻酸和聚赖氨酸等予以微囊化。每个微囊化和未微囊化胰岛体外培养每24h释放的胰岛素分别为63±42μu和69±42μu。7只链脲霉素诱发糖尿病大鼠不使用免疫抑制剂,接受一次性腹腔内移植(4.0～4.5)×10~3个微囊化胰岛后血糖恢复正常或接近正常(由19.4±1.6mmol/L降至7.9±3.4mmol/L)。每只受鼠每天的饮水量及尿量也明显下降。移植后血糖恢复正常的最长时间已超过222d,仍在继续随访。
Rat islets were isolated from the pancreas using a modified collagenase digestion technique and fibrin density gradient centrifugation, and microencapsulated with alginic acid, polylysine, and the like according to Sun et al. The release of insulin from each microencapsulated and non-microencapsulated islet in vitro was 63±42 μu and 69±42 μu, respectively. Seven streptozotocin-induced diabetic rats did not use immunosuppressive agents, and blood glucose returned to normal or nearly normal after a single intraperitoneal (4.0 to 4.5)×10 to 3 microencapsulated islets (from 19.4±1.6 mmol/kg). L decreased to 7.9±3.4 mmol/L). Daily water intake and urine output of each recipient mouse also decreased significantly. The longest post-transplant blood glucose return to normal has exceeded 222d, and follow-up continues.