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以芡实(Euryale ferox)叶片和茎段为外植体,采用MS培养基,附加2,4-D或NAA以及6-BA或KT,根据正交设计的原理,通过因子分析和聚类分析,研究不同激素配比对芡实一步成苗组培快繁的影响。结果表明:在芡实一步成苗快速繁殖中最佳的激素配比是MS+NAA0.2mg/L+6-BA 2.0mg/L,其余依次是MS+2,4-D 0.5mg/L+6-BA 1.0mg/L、MS+2,4-D0.2mg/L+6-BA 1.0mg/L、MS+2,4-D 1.0mg/L+KT 1.0mg/L、MS+NAA 1.5mg/L+6-BA1.0mg/L。
Using the leaves and stem segments of Euryale ferox as explants, MS medium supplemented with 2,4-D or NAA and 6-BA or KT was used to study the effects of different concentrations of 2,4- The effects of different hormone combinations on the tissue culture of Plectranthus ambrosioides in vitro were studied. The results showed that the best hormonal ratio of MS + NAA0.2mg / L + 6-BA 2.0mg / L was the best one in the process of rapid propagation of A. tumefaciens, followed by MS + 2,4-D 0.5mg / L + BA 1.0 mg / L, MS + 2,4-D 0.2 mg / L + 6-BA 1.0 mg / L, MS + 2,4-D 1.0 mg / L + KT 1.0 mg / /L+6-BA1.0mg/L.