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采用一种新颖的方法制备了过氧化物酶生物传感器.以铂盘电极为基底,电聚合普鲁士蓝(PB)电子媒介体,并在其表面覆盖一层三维溶胶凝胶膜,以防PB渗漏及利用其网状结构中的大量巯基吸附纳米金,最后利用纳米金静电吸附固定辣根过氧物酶制备过氧化氢传感器.通过循环伏安法对电极的修饰过程进行了表征,探讨了pH、温度对电极响应的影响.在优化的工作条件下,该传感器与H2O2浓度在7.0×10-6～6.6×10-3mol/L范围内呈线性关系,检出限为3.0×10-6mol/L.此外,该传感器具有较高的灵敏度,且能有效地消除抗坏血酸等的干扰.
A novel method was used to prepare peroxidase biosensor, which was electropolymerized with Prussian blue (PB) electron mediator based on platinum plate electrode and covered with a three-dimensional sol-gel film on its surface to prevent PB penetration Leakage and the use of a large number of its network structure of the adsorption of gold nanoparticles, and finally the use of gold nanoparticles electrostatically immobilized horseradish peroxidase hydrogen peroxide sensor prepared by cyclic voltammetry electrode modification process was characterized to explore pH and temperature on the electrode response.Under the optimized working conditions, the sensor has a linear relationship with the concentration of H2O2 in the range of 7.0 × 10-6 ~ 6.6 × 10-3mol / L with the detection limit of 3.0 × 10-6mol / L. In addition, the sensor has a high sensitivity, and can effectively eliminate ascorbic acid and other interference.