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目的探讨六味地黄颗粒对哮喘大鼠炎症介质白三烯β4(LTB4)、白三烯C4(LTC4)及5-脂氧合酶(5-LO)含量的影响。方法将SFP级SD大鼠160只随机分为8组,每组20只。除空白对照组和生理盐水组外,其余各组均以卵蛋白(OVA)辅以氢氧化铝凝胶为佐剂注射致敏,2周后雾化吸入2%OVA激发哮喘,建立哮喘模型,从实验第8天,各组分别用药干预,生理盐水组用生理盐水腹腔注射及吸入代替,空白对照组不予处理,造模时间共28d,取材后,高倍镜下观察各组大鼠肺及支气管的病理学改变;采用ELISA法检测肺组织匀浆中LTB4、LTC4的浓度。对大鼠肺组织中5-LO含量进行免疫组化评估。结果六味地黄颗粒组、六味地黄颗粒辅助地塞米松组、六味地黄颗粒辅助布地奈德组肺组织中LTB4、LTC4的浓度明显低于哮喘模型组(P<0.05)。各用药组肺组织中5-LO含量与哮喘模型组比较并无明显差异(P>0.05)。结论六味地黄颗粒在治疗哮喘过程中可能抑制肺组织中LTB4、LTC4分泌,这可能是其治疗哮喘的作用靶点之一。
Objective To investigate the effect of Liuwei Dihuang granule on the levels of inflammatory mediators LTB4, LTC4 and 5-LOX in asthmatic rats. Methods 160 SFP SD rats were randomly divided into 8 groups of 20 rats. Except blank control group and saline group, all the other groups were sensitized with aluminum hydroxide gel adjuvant with ovalbumin (OVA) supplemented with 2% OVA inhalation after 2 weeks, asthma model was established, From the 8th day of the experiment, the rats in each group were given drug intervention respectively. The saline group was treated by intraperitoneal injection of saline and inhalation instead of blank control group. The modeling time was 28 days. Bronchial pathological changes; using ELISA method to detect lung homogenate LTB4, LTC4 concentration. Immunohistochemical evaluation of 5-LO content in rat lung tissue was performed. Results The concentrations of LTB4 and LTC4 in Liuweidihuang granule group, Liuwei Dihuang granule-assisted dexamethasone group and Liuweidihuang granule-assisted budesonide group were significantly lower than those in asthma model group (P <0.05). There was no significant difference in the 5-LO content in the lung tissue of each drug-treated group compared with the asthma model group (P> 0.05). Conclusion Liuweidihuang granule may inhibit the secretion of LTB4 and LTC4 in lung tissue during the treatment of asthma, which may be one of the target of its treatment of asthma.