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采用sephadex G-50F柱层析及CM-sephadex C-50离子交换柱梯度洗脱法,从峰毒中分出Ⅰ、Ⅱ、Ⅲ及Ⅳ组分。 全峰毒对小白鼠的LD50为4.32mg/kg(3.91～4.76mg/kg)。全蜂毒对电刺激小白鼠的痛嘶叫反应有明显的抑制作用,2.16mg/kg剂量的抑制率达90％(P<0.05),1.44mg/kg的为55％。全蜂毒及组分Ⅱ的镇痛作用有明显的量—效关系及时—效关系。以小鼠热板法测定的全蜂毒的ED50为1.20mg/kg;组分Ⅱ为1.02mg/kg。1小时起效,5小时达高峰,持续12～48小时。但组分Ⅱ的作用时间较短。全蜂毒明显抑制致炎剂引起的炎症反应(P<0.001)。此作用有量效关系。同时,亦明显抑制化学性致热反应。
Sephadex G-50F column chromatography and CM-sephadex C-50 ion exchange column gradient elution were used to separate the I, II, III, and IV components from the peak toxicity. The LD50 of the whole peak poisoning to mice was 4.32 mg/kg (3.91 to 4.76 mg/kg). The whole bee venom has obvious inhibitory effect on the electrical stimuli in the mice. The inhibition rate of the 2.16 mg/kg dose is 90% (P<0.05), and that of the 1.44 mg/kg is 55%. The analgesic effect of whole bee venom and component II has a significant dose-effect relationship in time-effect relationship. The ED50 of whole bee venom measured by the mouse hot plate method was 1.20 mg/kg; component II was 1.02 mg/kg. 1 hour onset, 5 hours peak, lasting 12 to 48 hours. However, the action time of component II is shorter. Whole bee venom significantly inhibited the inflammatory response induced by the inflammatory agent (P<0.001). This effect has a dose-effect relationship. At the same time, it also significantly inhibited the chemically induced heat reaction.