Inductively coupled plasma emission spectroscopic and flame photometric analysis of goat epididymal

来源 :Asian Journal of Andrology | 被引量 : 0次 | 上传用户:shmilygang8751
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Aim: The elemental composition of the epididymal luminal fluid (ELF) in adult goat ( Capra indica) was investigat-ed. Methods: ELF was collected by micropuncture from twelve sites along the epididymal duct. The elementalcontents was analyzed with inductively coupled plasma (ICP) emission spectroscopy, a microanalytical technique thatcan simultaneously measure many elements in minute volumes of sample. The Na and K concentrations were determinedby flame photometry. Results: ICP spectroscopy showed the presence of copper, calcium, nickel, iron, magne-sium, chromium, titanium and zinc in ELF, with fluctuating levels at different sites along the length of the epididymis.Cadmium, cobalt, lead and manganese were not fotmd. The Na~+ / K~+ ratio was seen to be higher at the initial seg-ments of the epididymis and lower at the distal. Conclusion: It is proposed that the observed characteristic distribu-tion of elements in ELF may have far reaching implications in sperm maturation and storage known to occur in th Aim: The elemental composition of the epididymal luminal fluid (ELF) in adult goat (Capra indica) was investigat-ed. Methods: ELF was collected by micropuncture from twelve sites along the epididymal duct. The elementalcontents were analyzed with inductively coupled plasma Results: ICP spectroscopy showed the presence of copper, calcium, nickel, iron, magne-sium, chromium, titanium and zinc in ELF, with fluctuating levels at different sites along the length of the epididymis. Cadmium, cobalt, lead and manganese were not fotmd. The Na ~ + / K ~ + ratio was seen to be higher at the initial segmetants of the epididymis and lower at the distal. Conclusion: It is proposed that the observed characteristic distribu- tion of elements in ELF may have far reaching implications in sperm maturation and storage kn own to occur in th
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