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以V(二氯甲烷)/V(N,N-二甲基甲酰胺)=4∶1为溶剂,通过电纺制备直径为(650±60)nm,孔径为96 nm×72 nm聚乳酸(PLLA)纳米孔纤维膜。利用氧等离子体处理将亲水性单体丙烯酸(AA)接枝到纤维表面制备聚乳酸接枝丙烯酸(PLLAg-PAA)纳米孔纤维膜。与PLLA纳米孔纤维膜相比,PLLA-g-PAA纳米孔纤维膜的水接触角从(119.4±1.2)°降低到(42.3±0.6)°,拉伸强度、杨氏模量和断裂伸长率略有降低。将牙髓干细胞(DPSCs)在纤维膜支架上培养,细胞生长密度顺序为PLLA-g-PAA纳米孔纤维膜>PLLA纳米孔纤维膜>PLLA纤维膜。由于PLLA-g-PAA纳米孔纤维膜表面粗糙,比表面积大,孔隙率高和表面亲水性好,更有利于细胞的粘附、迁移、分化和繁殖。PLLA-g-PAA纳米孔纤维膜有望成为优良的组织工程支架材料。
(650 ± 60) nm, 96 nm × 72 nm polylactic acid with a diameter of 96 nm × 72 nm was prepared by electrospinning with V (methylene chloride) / V (N, N-dimethylformamide) PLLA) nanoporous fiber membrane. The polylactic acid grafted acrylic acid (PLLAg-PAA) nanoporous fiber membrane was prepared by grafting hydrophilic monomer acrylic acid (AA) onto the fiber surface by oxygen plasma treatment. The water contact angle of the PLLA-g-PAA nanoporous fibrous membranes decreased from (119.4 ± 1.2) ° to (42.3 ± 0.6) °, tensile strength, Young’s modulus and elongation at break compared to PLLA nanoporous fibrous membranes Rate slightly lower. Dental pulp stem cells (DPSCs) were cultured on the fibrous scaffolds. The order of cell growth was PLLA-g-PAA nanoporous membrane> PLLA nanoporous membrane> PLLA membrane. PLLA-g-PAA nanoporous fiber membrane surface roughness, large specific surface area, high porosity and surface hydrophilic, more conducive to cell adhesion, migration, differentiation and reproduction. PLLA-g-PAA nanoporous fiber membrane is expected to become excellent tissue engineering scaffold material.