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1.用~3H-5-羟色胺脑室灌流清醒动物的方法,测定了脑内5-羟色胺的释放。实验结果表明,给兔静注咪达氟(4mg/kg)后,因动物挣扎引起5-羟色胺释放加强,可能咪达氟不直接加强兔脑5-羟色胺的释放。因此,必须指出,在用清醒动物脑室灌流的方法研究药物对神经递质释放是否有影响时,必须考虑到清醒动物的活动状态。2.用荧光方法测定了多巴胺含量。实验结果表明,给兔静注眯达氟(4或8mg/kg)以后10分钟,颈部和前肢产生明显肌松时海马中多巴胺含量虽有增加的趋势,但无统计学意义,尾核中多巴胺含量基本不变。因此,可以认为咪达氟所引起的肌肉松弛可能与脑内多巴胺含量关系不大。
Serum 5-HT release was measured by perfusion of ~ 3H-5-HT into conscious animals. Experimental results show that intravenous imidafluoride (4mg / kg) intravenous infusion of 5-hydroxytryptamine is intensified due to animal struggles. It may be that imidafluoride does not directly enhance the release of 5-hydroxytryptamine in rabbit brain. Therefore, it must be pointed out that in conscious animal ventricular perfusion method to study the release of neurotransmitters drugs have an impact, we must take into account the activity of conscious animals. 2. Fluorescence method was used to determine dopamine content. The experimental results showed that although the dopamine content in the hippocampus of the rabbits tended to be increased at 10 minutes after intravenous injection of either rifampicin (4 or 8 mg / kg), there was no significant difference in the caudate nucleus Dopamine content remained unchanged. Therefore, it can be considered middl-induced muscle relaxation may have little effect on brain dopamine content.