论文部分内容阅读
人mes试验是应用组氨酸缺陷型鼠伤寒沙门氏菌作为遗传指示物的一种回复突变试验,因而,在培养基中,只允许加入痕量组氨酸,否则,活菌得以生长,影响获得正确结果。然而,某些食品常富含包括游离组氨酸在内的各种氨基酸。测定血、尿样品时,也会遇到组氨酸的干扰。因此,检测此类样品,须去除样品中所含的组氨酸。我们应用非极性树脂XAD-2处理了两个含组氨酸的样品,即高蛋白营养冲剂和“地龙液”。它们在经XAD-2滤过前的组氨酸含量分别为30.13mg/100ml和29.00mg/100ml。滤后为0.49mg/100ml和0.7mg/100ml去除率达97.6％和98.4％,而残留率仅1.6％和2.4％,符合可直接进行Ames试验的要求。
The human mesothelium test is a back-mutation test using histidine-deficient Salmonella typhimurium as a genetic indicator so only trace amounts of histidine can be added to the medium, otherwise the viable cells can grow and the effect is correctly obtained result. However, some foods are often rich in various amino acids, including free histidine. Determination of blood, urine samples, will also encounter histidine interference. Therefore, to detect such samples, the histidine contained in the sample must be removed. We applied two non-polar resin XAD-2 treated histidine-containing samples, namely high-protein nutritional granules and “earthworm.” Their histidine content before XAD-2 filtration was 30.13 mg / 100 ml and 29.00 mg / 100 ml, respectively. After filtration, the removal rates of 0.49mg / 100ml and 0.7mg / 100ml were 97.6% and 98.4%, while the residual rates were only 1.6% and 2.4%, respectively, which met the requirements of Ames test.