实验研究了超声波光生物制氢反应器的启动工艺以及反应器的产氢特性，探讨了超声时间和超声功率对反应器产氢性能的影响。超声波光生物反应器的启动实验进行了96h，此时反应器光合细菌生物量和反应液pH值趋于稳定，启动完成。在系统稳定运行后，随着超声时间、超声功率的增大，超声波光生物制氢反应器的产氢速率和产氢得率呈先增加后降低的趋势，然而葡萄糖去除率却随着超声时间的增加而增大。“,”Experimental study on the start-up process and hydrogen production in an ultrasonic photo-biological reactor were conducted in the present study and the effects of ultrasonic time and ultrasonic output power on hydrogen production performance of the bioreactor were investigated. The biomass and pH value of the culture were adopted as indexes to evaluate the start-up process of the bioreactor, and they kept stable after 96 h of start-up, which indicated the start-up process succeeded. In the stable operation stage, the hydrogen production rate and hydrogen yield of the ultrasonic bioreactor firstly increased and then dropped with increases in the ultrasonic time and output power, respectively. However, the glucose removal rate always increased with increasing ultrasonic time.