论文部分内容阅读
目的:研究缺氧对人肝癌细胞MHCC-97L迁移的影响,并初步揭示其分子机制。方法:建立划痕实验检测缺氧条件下人肝癌细胞MHCC-97L迁移能力变化的可靠模型,研究Wnt5a对MHCC-97L细胞迁移的调控。结果:缺氧使MHCC-97L细胞缺氧诱导因子1α(hypoxia-inducible factor-1α,HIF-1α)和Wnt5a mRNA表达上调,并可促进MHCC-97L细胞迁移能力显著增强,干扰HIF-1α表达的MHCC-97L细胞在缺氧条件下Wnt5a mRNA表达显著降低,干扰Wnt5a表达的MHCC-97L细胞在缺氧环境中的迁移能力则明显降低。结论:在缺氧条件下MHCC-97L细胞迁移能力可发生明显改变,HIF-1α和Wnt5a调控其迁移过程。这些结果为深入阐明肝癌细胞转移和侵袭分子调控机制提供了新的实验线索。
Objective: To study the effect of hypoxia on the migration of human hepatocellular carcinoma cell line MHCC-97L and to reveal its molecular mechanism. METHODS: A reliable model for the detection of migration of human hepatocellular carcinoma cell line MHCC-97L under hypoxic conditions was established by scratch testing to study the regulation of Wnt5a on migration of MHCC-97L cells. RESULTS: Hypoxia increased the expression of hypoxia-inducible factor-1α (HIF-1α) and Wnt5a mRNA in MHCC-97L cells, and promoted the migration of MHCC-97L cells, and interfered with the expression of HIF-1α. The expression of Wnt5a mRNA was significantly decreased in MHCC-97L cells under hypoxic conditions, and the migration ability of MHCC-97L cells that interfered with Wnt5a expression in hypoxic conditions was significantly reduced. CONCLUSION: The migration ability of MHCC-97L cells can be significantly changed under hypoxic conditions. HIF-1α and Wnt5a regulate their migration. These results provide new experimental clues for further elucidating the mechanism of metastasis and invasion of liver cancer cells.