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目的:探讨RNA干涉E-cadherin表达对人乳腺癌MCF-7细胞紫杉醇化疗敏感性的影响,探讨可能的分子机制。方法:通过慢病毒介导的E-cadherin干涉RNA感染乳腺癌MCF-7细胞,MTT法检测紫杉醇对乳腺癌MCF-7细胞的生长抑制作用,流式细胞术检测细胞凋亡和细胞周期,RT-PCR检测Bcl-2、Bax和E-cadherin mRNA表达变化。结果:E-cadherin干涉RNA感染后,乳腺癌MCF-7细胞对紫杉醇作用下的生长抑制率和细胞凋亡率均显著降低。与对照相比,凋亡诱导时E-cadherin干涉组细胞中Bcl-2 mRNA表达显著升高,E-cadherin mRNA表达显著降低。结论:E-cadherin表达抑制显著降低了乳腺癌细胞对紫杉醇化疗的敏感性,其凋亡影响机制可能与Bcl-2表达上调有关。
OBJECTIVE: To investigate the effect of E-cadherin RNA interference on paclitaxel chemosensitivity in human breast cancer MCF-7 cells and to explore the possible molecular mechanisms. Methods: The breast cancer MCF-7 cells were infected by lentivirus-mediated E-cadherin interference RNA. The growth inhibition of paclitaxel on breast cancer MCF-7 cells was detected by MTT assay. The apoptosis and cell cycle were detected by flow cytometry. -PCR detection of Bcl-2, Bax and E-cadherin mRNA expression changes. RESULTS: After E-cadherin interference with RNAi, the growth inhibition rate and apoptosis rate of breast cancer MCF-7 cells were significantly decreased under paclitaxel treatment. Compared with the control, the expression of Bcl-2 mRNA and the expression of E-cadherin mRNA in E-cadherin intervention group were significantly decreased after apoptosis induction. Conclusion: Inhibition of E-cadherin expression significantly reduces the sensitivity of breast cancer cells to paclitaxel chemotherapy. The mechanism of apoptosis may be related to the up-regulation of Bcl-2 expression.