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[目的]探讨线粒体DNA控制区微卫星不稳定性(mitochondrial microsatellite instability,mtMSI)与肺癌的相关性。[方法]采用PCR-SSCP(PCR single-stranded conformation polymorphism,PCR-SSCP)和序列测定技术对37例肺癌组织及癌旁组织线粒体DNA控制区的3个微卫星位点进行了分析。[结果]37例肺癌样本中共检出mtMSI12例(32.4%),肺癌mtMSI发生率与患者性别、年龄及肿瘤的病理类型无相关性(P>0.05),吸烟组(包括曾经吸烟者)与非吸烟组mtMSI发生率两者有显著差异(P<0.05)。[结论]线粒体DNA控制区的遗传不稳定性在一些肺癌的发生中可能起一定作用。
[Objective] To explore the relationship between mitochondrial microsatellite instability (mtMSI) and lung cancer in mitochondrial DNA control region. [Methods] Three microsatellite loci in the mitochondrial DNA control region of 37 lung cancer tissues and paracancerous tissues were analyzed by PCR-SSCP (PCR single-stranded conformation polymorphism, PCR-SSCP) and sequencing techniques. [Results] Thirty-six cases (32.4%) of mtMSI were detected in 37 lung cancer samples. There was no correlation between the incidence of lung cancer mtMSI and the sex, age, and pathological type of the tumor (P>0.05). The smoking group (including former smokers) and non- The incidence of mtMSI in the smoking group was significantly different (P<0.05). [Conclusion] The genetic instability of mitochondrial DNA control region may play a role in the occurrence of some lung cancers.