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目的:探讨油茶皂苷在体外诱导人白血病Jurkat细胞的凋亡及其可能机制。方法:将不同浓度的油茶皂苷作用于人白血病Jurkat细胞,应用细胞计数法检测其对细胞增殖的影响,FCM检测细胞周期分布和细胞凋亡率,蛋白质印迹法检测细胞caspase-3、多聚ADP-核糖聚合酶[poly(ADP-ribose)polymerase,PARP]、p-Bcl-2、Bcl-2、Bax和caspase-9蛋白的表达。结果:1～16μg/mL油茶皂苷可抑制Jurkat细胞的增殖,呈剂量依赖性。1～4μg/mL油茶皂苷作用Jurkat细胞24h后,G0/G1期和G2/M期细胞比率下降,S期细胞比率上升,细胞凋亡率随着油茶皂苷浓度的增加而上升;Jurkat细胞中,p-Bcl-2和Bcl-2蛋白的表达水平下调,caspase-3、PARP和caspase-9蛋白的表达水平上调,Bax蛋白的表达水平无明显变化。结论:油茶皂苷能抑制人白血病Jurkat细胞增殖和诱导其凋亡,其作用机制可能与细胞凋亡的线粒体途径有关。
Objective: To investigate the apoptosis of Jurkat cells induced by Camellia oleifera saponins in vitro and its possible mechanism. Methods: Different concentrations of Camellia saponin were applied to human leukemia Jurkat cells. The cell proliferation was measured by cell counting. The cell cycle distribution and apoptosis rate were detected by FCM. The expressions of caspase-3, poly ADP - Expression of poly (ADP-ribose) polymerase, PARP, p-Bcl-2, Bcl-2, Bax and caspase-9 proteins. Results: 1 ~ 16μg / mL Camellia saponin could inhibit the proliferation of Jurkat cells in a dose-dependent manner. After Jurkat cells were treated with 1 ~ 4μg / mL Camellia saponin for 24 hours, the percentage of cells in G0 / G1 phase and G2 / M phase decreased and the percentage of cells in S phase increased. The apoptosis rate of Jurkat cells increased with the increase of saponin concentration in Jurkat cells. The expressions of caspase-3, PARP and caspase-9 were up-regulated while the expressions of Bcl-2 and Bcl-2 were down-regulated. Conclusion: Camellia saponin can inhibit the proliferation and induce the apoptosis of human leukemia Jurkat cells. The mechanism may be related to the mitochondrial pathway of apoptosis.