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目的：建立环境致癌（突变）物和药物代谢相关的人细胞色素Ｐ４５０１Ａ２，２Ａ６，２Ｅ１，３Ａ４ｃＤＮＡ的克隆和转基因细胞系。方法：从人肝组织中提取总ＲＮＡ，用逆转录酶生成ｃＤＮＡ。以各个ｃＤＮＡ编码序列两端设计引物，ＰＣＲ扩增各个人细胞色素Ｐ４５０基因的ｃ?
OBJECTIVE: To establish a cloning and transgenic cell line of human cytochrome P4501A2, 2A6, 2E1, 3A4 cDNA related to drug metabolism and environment carcinogens (mutants). Methods: Total RNA was extracted from human liver tissue and cDNA was generated using reverse transcriptase. Primers were designed at both ends of each cDNA coding sequence to amplify c?