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布鲁氏菌ery操纵子参与赤藓醇代谢.赤藓醇能够促进布鲁氏菌的生长.为进一步研究布鲁氏菌引发宿主流产的分子机制,采用基因重组技术构建布鲁氏菌ery操纵子启动子缺失株(△ery),通过体内外实验探讨布鲁氏菌ery操纵子的生物学功能.研究结果显示,获得了布鲁氏菌ery操纵子缺失株;布鲁氏菌ery操纵子缺失株侵染胚胎滋养层细胞脱落较亲本株明显下降;巨噬细胞CFU计数缺失株作用组和亲本株作用组差异显著(P<0.05).试管凝集和虎红平板实验结果显示均出现凝集现象;检测血清中细胞因子IL-10和TNF-α的表达水平,△ery诱导机体产生的IL-10和TNF-α明显低于亲本株(P<0.05).小鼠脾脏细菌CFU计数结果显示,△ery较亲本株毒力明显下降.本研究表明,布鲁氏菌ery操纵子启动子缺失株毒力较亲本株明显下降,为进一步揭示布鲁氏菌引起流产的致病机制提供了一定的理论依据.
Brucella ery operon involved in erythritol metabolism.Erythritol can promote the growth of brucella.For further study on the molecular mechanism of brucellosis-induced host abortion, gene recombination technology is used to construct brucella erythrooperation (△ ery), in vitro and in vivo experiments to explore the biological function of erythrocytes of brucella bacteria erythropoiesis.The results showed that the Brucella eryanillosomal deletion strain was obtained; Brucella ery operon Deletion of infected trophoblast cells inoculated compared with the parental strain decreased significantly; macrophage CFU count deletion group and the role of the parent group significant difference (P <0.05) .Thin tube agglutination and tiger red plate test results show that the phenomenon of aggregation The level of IL-10 and TNF-α in serum was detected, and the level of IL-10 and TNF-α produced by △ ery was significantly lower than that of the parental strain (P <0.05) △ ery than the virulence of virulence significantly decreased.This study shows that Brucella erythel operon promoter deletion virulence decreased significantly than the parental strain, in order to further reveal the pathogenesis of Brucella caused miscarriage has provided some Theoretical basis.