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目的 :评价免疫组化双重染色在毛细淋巴管研究中的作用。方法 :利用荆豆凝集素及IV型胶原单克隆抗体分别标记内层细胞和基底膜 ,对舌、颊等组织进行免疫组化双重染色。结果 :毛细淋巴管由于仅有一层内皮细胞构成而呈单层着色 ,而毛细血管则由于内皮细胞外还有一层完整的基底膜而呈双层着色。结论 :免疫组化双重染色法在毛细淋巴管的研究中是一种较为准确、客观的方法。
Objective: To evaluate the role of immunohistochemical double staining in the study of lymphatic capillaries. Methods: The inner layer cells and the basement membrane were labeled with monoclonal antibody of Vitexin type IV and type IV collagen, respectively. The tongue, cheek and other tissues were double-stained immunohistochemically. RESULTS: Capillary lymphatic vessels were monolayer colored with only one layer of endothelial cells and capillary vessels were double-stained with a complete basement membrane outside of endothelial cells. Conclusion: Immunohistochemical double staining is a more accurate and objective method in the study of lymphatic capillaries.