本实验分为实验组和对照组,实验组对钛片进行微弧氧化(MAO)处理,对照组仅对钛片进行抛光处理。采用电镜观察试样表面形貌,将MG63细胞与钛材复合培养,通过MTT方法检测成骨细胞增殖,并测定碱性磷酸酶(Alkaline phosphatase,ALP)活性。通过蛋白质印迹法(Western-blot)检测各组成骨细胞的成骨相关蛋白:I型胶原(Collagen-I,Col-I)、骨钙素(osteocalcin,OCN)、骨桥蛋白(osteopontin,OPN)、runt相关转录因子2(runt-related transcription factor-2, The experiment was divided into experimental group and control group, the experimental group of micro-oxidation of titanium (MAO) treatment, the control group only polished titanium. The morphology of the sample was observed by electron microscope. MG63 cells and titanium were cultured in vitro. The proliferation of osteoblasts was detected by MTT assay and the activity of alkaline phosphatase (ALP) was measured. The osteogenesis-related proteins (Collagen-I, Col-I, OCN, OPN) of osteoblasts were detected by Western-blot. , Runt-related transcription factor-2