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目的在感染的8只恒河猴的SARS-CoV模型动物中,观察肺等组织中出现的系列病理学改变,为针对抗SARS药物筛选、疫苗评价中的免疫病理反应等奠定实验依据。方法SARS-CoV经鼻腔接种8只恒河猴,在感染的第5、7、10、15、20、30和60天,分别安乐处死动物,组织病理取材,制片,观察。结果经病毒分离和RT-PCR证实动物感染是成功的。系列病理改变表明,早期肺组织可见间质性肺炎,水肿、结构破坏、出血,巨噬细胞浸润;后期出现内皮细胞受损及再生,透明膜形成,小血管玻璃样变,肺组织纤维化及肺气肿形成,肺泡网状纤维和弹力纤维破坏并增生等,脾脏、淋巴结生发中心早期有萎缩,后期有恢复等病理学改变均和SARS患者相似。结论感染恒河猴出现与SARS患者类似的临床和病理学改变,为进一步研究该病毒的病原特性、发病机理、药物筛选、疫苗评价等方面的研究奠定了重要基础。
Objective To observe the pathological changes in lung and other tissues of SARS-CoV model in 8 infected rhesus monkeys to establish the experimental basis for the screening of anti-SARS drugs and the immunopathological response in vaccine evaluation. Methods Eight Rhesus monkeys were inoculated intranasally with SARS-CoV. Animals were sacrificed on days 5, 7, 10, 15, 20, 30 and 60, respectively. Results Animal infection was confirmed by virus isolation and RT-PCR. A series of pathological changes showed that interstitial pneumonia, edema, structural destruction, hemorrhage and macrophage infiltration were observed in early lung tissue. Endothelial cell injury and regeneration, hyaline membrane formation, small vessel glassy transition, lung fibrosis and Emphysema formation, alveolar reticular fibers and elastic fibers destroyed and hyperplasia, spleen, lymph node germinal center early atrophy, postoperative recovery and other pathological changes are similar to patients with SARS. Conclusions The clinical and pathological changes of rhesus monkeys infected with SARS were similar, which laid an important foundation for further study on the pathogenicity, pathogenesis, drug screening and vaccine evaluation of the virus.