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目的:观察17-甲氧基-7-羟基-苯并呋喃查尔酮(YLSC)对垂体后叶素(Pit)致心肌缺血大鼠的影响。方法:将70只SD大鼠随机分为7组,每组10只:正常对照组,模型对照组,溶媒组,阳性药对照组,YLSC低、中、高剂量组(1.25,2.50,5.00 mg.kg-1)。各组尾静脉给予相应药物后,观察垂体后叶素致心肌缺血大鼠Ⅱ导联心电图的变化,伊文思蓝和三苯基氯化四氮唑(TTC)双重染色确定心肌缺血面积,HE染色观察心肌形态学变化,Elisa法检测血清生化标志物MB型肌酸激酶(CK-MB)、肌钙蛋白(cTnI)和肌红蛋白(MyoG)的含量。结果:YLSC能剂量依赖性的减少大鼠15 s,30 s,1 min,5 min的ST段抬高,与模型组比较,差异有显著性(P<0.05或P<0.01);同时能剂量依赖性的缩小模型组心肌缺血面积(P<0.05或P<0.01),减少心肌标志物CK-MB,cTnI,MyoG的漏出(P<0.05或P<0.01),改善心肌水肿、出血和炎细胞渗出的状况。结论:YLSC对垂体后叶素所致心肌缺血大鼠有明显保护作用。
Objective: To observe the effect of 17-methoxy-7-hydroxy-benzofuran chalcone (YLSC) on pituitary-induced myocardial ischemia in rats. Methods: Seventy SD rats were randomly divided into 7 groups with 10 rats in each group: normal control group, model control group, vehicle group, positive drug control group, YLSC low, medium and high dose groups (1.25, 2.50, 5.00 mg .kg-1). After the caudal vein was administrated with the corresponding drugs in each group, the changes of electrocardiogram (ECG) of Ⅱ lead of vasopressin-induced myocardial ischemia rats were observed. The myocardial ischemia area was determined by Evans blue and triphenyltetrazolium tetrazolium (TTC) The myocardial morphological changes were observed. Elisa method was used to detect the levels of MB-MB, cTnI and MyoG. Results: YLSC reduced ST-segment elevation at 15, 30, 1, and 5 min in a dose-dependent manner in rats. Compared with the model group, YLSC showed significant difference (P <0.05 or P <0.01) Decreased myocardium ischemia area (P <0.05 or P <0.01) and decreased the leakage of myocardial markers CK-MB, cTnI and MyoG (P <0.05 or P <0.01), and improved myocardial edema, Exudation of cells. Conclusion: YLSC has significant protective effect on vasopressin-induced myocardial ischemia in rats.