单独感染HCV与HCV/HIV共感染患者外周血T淋巴细胞分化分析

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目的探讨单独感染HCV与HCV/HIV共感染患者外周血T淋巴细胞分化情况。方法采集单独感染HCV患者(12例)、HCV/HIV共感染患者(27例)以及正常人群(13例)的外周静脉血,检测ALT、AST等生化指标;提取外周血单个核细胞(PBMC),通过流式细胞检测技术检测CD4+T和CD8+T细胞比例。结果 HCV/HIV共感染患者ALT水平[(53.08±9.78)U/L]显著高于单独感染HCV患者[(26.22±4.25)U/L],差异有统计学意义(P=0.0064)。处于中期分化状态的CD4+T细胞,在正常人群中所占比例为(35.13±3.24)%,显著高于单独感染HCV患者[(9.01±3.70)%]和HCV/HIV共感染患者[(8.10±2.14)%],差异有统计学意义(P<0.001)。正常人群、单独感染HCV患者与HCV/HIV共感染患者晚期分化的CD4+T细胞所占比例分别为(2.52±2.54)%、(14.30±9.85)%及(15.23±11.58)%,正常人群晚期分化的CD4+T细胞比例显著高于单感染HCV与HCV/HIV共感染患者的比例,差异有统计学意义(P<0.05),单独感染HCV与HCV/HIV共感染患者之间无差异。正常人群处于早期分化和中期分化的CD8+T细胞的比例分别为(42.73±2.08)%、(36.17±2.85)%,均显著高于单独感染HCV患者同期水平[(28.27±4.36)%、(13.15±4.23)%]及HCV/HIV共感染患者[(18.02±2.01)%、(16.60±3.06)%],差异均有统计学意义(P<0.05);单独感染HCV患者的早期分化CD8+T细胞比例也显著高于HCV/HIV共感染患者,差异有统计学意义(P<0.05)。而处于晚期分化的CD8+T细胞,正常人群所占比例为(12.02±1.54)%,显著低于单独感染HCV患者[(49.30±3.85)%]及HCV/HIV共感染患者[(54.23±2.58)%],差异有统计学意义(P<0.05)。结论单独感染HCV及HCV/HIV共感染影响T淋巴细胞的分化状态;HCV/HIV共感染影响处于早期分化的CD8+T细胞比例。 Objective To investigate the differentiation of T lymphocytes in peripheral blood of patients infected with HCV and HCV / HIV alone. Methods Peripheral venous blood was collected from patients with HCV infection (12 cases), HCV / HIV co-infected patients (27 cases) and normal people (13 cases). ALT, AST and other biochemical parameters were detected. Peripheral blood mononuclear cells (PBMCs) Flow cytometric detection of CD4 + T and CD8 + T cell ratio. Results The level of ALT in HCV / HIV co-infected patients was significantly higher than that in HCV infected patients ([(53.08 ± 9.78) U / L] (26.22 ± 4.25 U / L, P = 0.0064). The percentage of CD4 + T cells in metaphase differentiation was (35.13 ± 3.24)% in the normal population, which was significantly higher than that in HCV infected patients (9.01 ± 3.70%) and HCV / HIV co-infected ± 2.14)%], the difference was statistically significant (P <0.001). The proportion of late-differentiated CD4 + T cells in patients with HCV infection and those infected with HCV / HCV alone was (2.52 ± 2.54)%, (14.30 ± 9.85)% and (15.23 ± 11.58)%, respectively, in the normal population The proportion of differentiated CD4 + T cells was significantly higher than that of single HCV and HCV / HIV co-infected patients (P <0.05). There was no difference between HCV infected and HCV / HIV co-infected patients. The proportion of CD8 + T cells in early and mid-term differentiation in normal population were (42.73 ± 2.08)% and (36.17 ± 2.85)%, respectively, which were significantly higher than those in HCV infected patients [(28.27 ± 4.36)% vs 13.15 ± 4.23%] and HCV / HIV co-infected patients [(18.02 ± 2.01)%, (16.60 ± 3.06)%], respectively. The difference was statistically significant T cell ratio was also significantly higher than HCV / HIV co-infected patients, the difference was statistically significant (P <0.05). The proportion of CD8 + T cells in advanced stage was (12.02 ± 1.54)%, which was significantly lower than that in HCV infected patients (49.30 ± 3.85%) and HCV / HIV co-infected patients (54.23 ± 2.58% )%], The difference was statistically significant (P <0.05). Conclusion The co-infection of HCV and HCV / HIV alone affects the differentiation of T lymphocytes. The co-infection of HCV / HIV affects the proportion of CD8 + T cells in early differentiation.
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