综合运用各种色谱分离技术对大叶桉叶乙醇提取物的正丁醇萃取部位进行化学成分研究,利用各种波谱技术结合理化常数和化学方法确定化合物的结构。该研究从中分离得到6个黄酮苷类成分,分别鉴定为(-)-2S-8-甲基-5,7,4’-三羟基二氢黄酮-7-O-β-D-吡喃葡萄糖苷(1)、槲皮素-3-O-(2″-没食子酰基)-α-L-阿拉伯糖苷(2)、山柰酚-3-O-α-L-阿拉伯糖苷(3)、番石榴苷(4)、三叶豆苷(5)和金丝桃苷(6)。其中,化合物1为1个新的黄酮苷,2~6为首次从该植物中分离得到。 Various chromatographic techniques were used to study the chemical constituents of n-butanol extraction of Eucalyptus grandis leaves ethanol extract. The chemical structures of the compounds were determined by various spectroscopic techniques combined with physico-chemical constants and chemical methods. Six flavonoid glycosides were isolated and identified as (-) - 2S-8-methyl-5,7,4’-trihydroxyflavone-7-O-β-D-glucopyranose Glycosides (1), quercetin -3-O- (2 "-galonyl) -α-L-arabinoside (2), kaempferol-3-O-α-L-arabinoside (4), tetrabutin (5) and hyperoside (6), of which compound 1 is a new flavonoid glycoside, and 2 ~ 6 are isolated from this plant for the first time.