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目的:研究原发性肝癌组织 AFP mRNA 的表达及意义。方法:采用逆转录 PCR 技术对31份原发性肝癌癌组织中 AFP mRNA 的表达进行了观察。结果:31份肝癌组织有24份(77.4%)检出 AFP mRNA,阳性者中有7例同时伴有癌旁肝组织 AFP mRNA 的表达,此7例患者均伴有明显的肝硬化。肝癌组织 AFP mRNA 检出率在血清 HBsAg 阳性组(86.9%),伴有肝硬化组(87.0%)和血清 AFP>30μg/L 组(100%)分别显著高于血清 HBsAg 阴性组(50%),无伴有肝硬化组(44.4%)和血清 AFP≤30μg/L 组(46.2%)(P 值<0.05～0.01)。结论:肝癌组织 AFP mRNA 的表达可能与 HBV 感染、肝细胞再生修复有关,检测肝硬化组织 AFP mRNA 表达可能有助于预测肝癌的早期发生。
Objective: To study the expression and significance of AFP mRNA in primary hepatocellular carcinoma. Methods: The expression of AFP mRNA in 31 HCC tissues was observed by reverse transcription PCR. RESULTS: AFP mRNA was detected in 24 (77.4%) of the 31 HCC tissues, and 7 of the positive cases were also associated with the expression of AFP mRNA in the para-tumor liver tissue. All of the 7 patients had cirrhosis. The detection rate of AFP mRNA in hepatocellular carcinoma was significantly higher in serum HBsAg positive group (86.9%), cirrhosis group (87.0%) and serum AFP>30 μg/L group (100%) than serum HBsAg negative group (50%) There was no associated cirrhosis group (44.4%) and serum AFP ≤ 30μg/L group (46.2%) (P value <0.05-0.01). Conclusion: The expression of AFP mRNA in hepatocellular carcinoma may be related to HBV infection and hepatocyte regeneration. The detection of AFP mRNA expression in liver cirrhosis may be helpful to predict the early occurrence of hepatocellular carcinoma.