论文部分内容阅读
目的:研究莨菪亭对人前列腺癌细胞PC_3增殖的作用和莨菪亭是否能引起PC_3细胞的凋亡。方法:用细胞生长曲线,MTT试验和酸性磷酸酶(ACP)活性来测定细胞增殖,考马斯亮蓝法测细胞内蛋白质的含量,光镜、透射电镜和荧光显微镜观察莨菪亭引起的形态学变化。用荧光显微镜和流式细胞仪确定凋亡率和细胞的周期分布。结果:莨菪亭对PC_3,PAA和Hela细胞的IC_(50)分别为(157±25),(154±51)和(294±100)mg/L,莨菪亭时间和浓度依赖性地抑制PC_3细胞的增殖,并引起细胞内蛋白质含量减少和ACP活性降低。经莨菪亭处理后,在光镜、透射电镜和荧光显微镜下可观察到莨菪亭引起的典型的凋亡形态学变化,流式细胞仪测定显示经莨菪亭0,100,200和400mg/L处理后PC_3细胞的凋亡率分别为0.3％,2.1％,9.3％和35％,G_2期细胞显著减少。结论:莨菪亭抑制PC_3细胞增殖且可引起PC_3细胞的凋亡。
Objective: To study the effect of scoptidine on the proliferation of human prostate cancer cell line PC_3 and whether scopolamine could induce the apoptosis of PC 3 cells. Methods: Cell proliferation was measured by cell growth curve, MTT assay and acid phosphatase (ACP) activity. Intracellular protein content was measured by Coomassie Brilliant Blue assay. Morphological changes were observed by light microscope, transmission electron microscope and fluorescence microscope. Fluorescence microscopy and flow cytometry were used to determine apoptotic rate and cell cycle distribution. Results: The IC50 of PC3, PAA and Hela cells were (157 ± 25), (154 ± 51) and (294 ± 100) mg / Proliferation, and cause a decrease in intracellular protein content and ACP activity. Typical morphological changes of scopolamine - induced apoptosis were observed under light microscope, transmission electron microscope and fluorescence microscope after treatment with scopolamine. Flow cytometry analysis showed that the cells treated with scopolamine at 0, 100, 200 and 400 mg / L of PC_3 cells The apoptosis rate was 0.3%, 2.1%, 9.3% and 35%, respectively. Conclusion: Scopolamine inhibits the proliferation of PC 3 cells and induces the apoptosis of PC 3 cells.