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目的研究骨髓间充质干细胞(BMSCs)向心肌细胞分化过程中离子通道电流的表达情况,为临床BM-SCs移植的安全性提供参考。方法分离培养BMSCs及乳鼠心肌细胞(NRVM s),并以1∶10的比例共培养,应用全细胞膜片钳技术记录共培养的BMSCs上离子通道电流的表达情况。结果成功分离培养BMSCs和NRVM s并建立了共培养模型,共培养1周后,BMSCs形态发生了明显变化;在心肌微环境诱导下,向心肌分化的BMSCs平均膜电位为-(40.37±3.68)mV,平均膜电容为(35.18±4.96)pF;共培养的BMSCs上可记录到IK1、Ito、IKsus,未记录到INa和ICa,也未记录到KCa。结论BMSCs和NRVM s共培养1周后,BMSCs上记录到IK1、Ito及IKsus,但未记录到INa和ICa。
Objective To study the expression of ion channel currents during the differentiation of bone marrow mesenchymal stem cells (BMSCs) into cardiomyocytes and to provide references for the safety of clinical BM-SCs transplantation. Methods BMSCs and neonatal rat cardiomyocytes (NRVMs) were isolated and cultured, and co-cultured in a ratio of 1:10. Whole-cell patch clamp technique was used to record the ion channel currents in co-cultured BMSCs. RESULTS: BMSCs and NRVMs were isolated and cultured successfully. After 1 week of co-culture, the morphology of BMSCs changed significantly. Under myocardial microenvironment, the average membrane potential of BMSCs differentiated into myocardium was (- 40.37 ± 3.68) mV and the average membrane capacitance was (35.18 ± 4.96) pF; IK1, Ito, IKsus were recorded on co-cultured BMSCs, no INa and ICa were recorded, and no KCa was recorded. Conclusions After co-cultured with BMSCs and NRVMs for 1 week, IK1, Ito and IKsus were recorded on BMSCs but not INa and ICa.