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神经白细胞素(NLK)是既作用于神经系统又作用于免疫系统的蛋白质(Mr 56 000),其表达调控于mRNA水平.150只SD纯系雄性大鼠分为对照组和冷暴露1,2,3,4周组.RNA打点杂交结果表明,大鼠冷暴露期间神经内分泌系统(垂体、下丘脑)和免疫系统(脾淋巴细胞)中 NLK mRNA的变化均一致,即RNA杂交点灰度比值:1周时增加到原值的1.63-11.31倍,2周时降低到原值的0.63倍,3周时再次增加到原值的1.92-21.09倍.这是对寒冷环境的一种普遍性反应.体外培养的淋巴细胞内合成蛋白质与上清中分泌蛋白质经SDS-PAGE放射自显影图谱分析表明淋巴细胞内NLK与其mRNA含量变化不呈平行关系,与对照组相比,冷暴露1周组NLK电泳条带极浅,而冷暴露2,3,4周组NLK放射自显影电泳条带明显增浓.这很可能是冷适应动物Ig合成增加的一个主要原因.
Neutrophil (NLK) is a protein that acts on both the nervous system and the immune system (Mr 56 000) and its expression is regulated at the mRNA level.150 SD male rats are divided into control group and cold exposure 1, 2 , 3 and 4 weeks.RNA RBI hybridization results showed that the changes of NLK mRNA in neuroendocrine system (pituitary, hypothalamus) and immune system (splenic lymphocytes) were consistent during the cold exposure in rats, that is, the gray ratio : 1 week increased to 1.63-11.31 times the original value, 2 weeks reduced to 0.63 times the original value, again increased to 1.92-21.09 times the original value at 3 weeks.This is a universal response to the cold environment The results of SDS-PAGE autoradiography showed that there was no parallel relationship between the expression of NLK and the mRNA level of lymphocytes in the lymphocytes and the secreted proteins in the supernatant. Compared with the control group, the expression of NLK The bands of electrophoresis were very light, while the band of NLK autoradiography was obviously enriched in groups 2, 3, and 4 after cold exposure, which is probably one of the main reasons for the increased Ig synthesis in cold-adapted animals.