论文部分内容阅读
目的 通过测定新疆地区静脉吸毒人群中HIV-1(1型人免疫缺陷病毒)感染者CD_4细胞、血浆细胞因子(IL-2,INF7,IL-4和IL-10)水平和末梢血单核细胞(PBMC)产生细胞因子的活性,了解该人群中HIV-1感染者细胞免疫学特征及其与病程的关系。方法 将36例HIV-1感染者分为2组,第(?)组CD_4细胞≥500/”l,第二组CD_4细胞<500/山l。CD_4细胞和细胞因子分别采用流氏细胞仪和ELISA方法测定。同时也检测了13例抗体阴性者血浆IL-2,INF~,IL-4和IL-10的含量,研究了6例感染者和6例抗体阴性者PBMC在体外产生IL-2和INF~的活性。结果 (1)与正常人比较,感染者血浆IL-2显著降低,且与疾病的严重程度呈正比(P<0.05);IFN7有升高趋势、IL-4和IL-10无显著升高;(2)感染者PBMC自发性产生IL-2的活性降低(P<0.05),但PHA刺激的IL-2的产生没有显著改变;而PBMC自发性及PHA刺激的IFN7的产生均较正常人显著降低(P<0.05)。结论 (1)新疆地区静脉吸毒人群中HIV-1感染者血浆IL-2降低与病程进展呈正相关,提示该因子的变化可以作为判断病程进展的指标;(2)HIV-1感染者血浆IL-2降低与其PBMC分泌该因子活性降低有直接关系;(3)IFNy有升高趋势、IL-4和IL-10无显著升高的结果均提示该人群中感染者尚处于病程的较早阶段。
Objective To determine the level of plasma cytokines (IL-2, INF7, IL-4 and IL-10) and the level of peripheral blood mononuclear cells (PBMCs) in HIV-1 infected human immunodeficiency virus type 1 (PBMC) cytokines activity, to understand the population of HIV-1 infected cells immunological characteristics and its relationship with the course. Methods Thirty-six HIV-1 infected patients were divided into two groups. The first group of CD4 + CD_4 cells ≥500 / "l, the second group of CD4 cells <500 / Hill l.CD4 cells and cytokines were flow cytometry and IL-2, INF-, IL-4 and IL-10 in 13 patients with negative antibody were also detected by ELISA.Among these 6 infected and 6 antibody-negative PBMC, IL-2 IL-2, IL-4 and IL-6 in the infected group were significantly lower than those in the normal group (P <0.05). The levels of IL-4 and IL- (P <0.05), but there was no significant change in PHA-stimulated IL-2 production. However, the spontaneous and PHA-stimulated IFN7 (P <0.05) .Conclusion (1) The decrease of plasma IL-2 level in HIV-1 infected patients in Xinjiang is positively correlated with the progression of the disease, suggesting that the changes of this factor can be used as a measure of the progression of the disease (2) There was a direct relationship between the decrease of plasma IL-2 in HIV-1 infected patients and the decrease of PBMC secretion of this factor; (3) The increase of IFNy and the increase of IL-4 and IL-10 It shows the population infected person is still in the early stages of the course.