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目的:研究雌激素干预对急性一氧化碳(CO)中毒迟发性脑病(DNS)的防治作用,探索有效的临床治疗手段。方法:选择成年Wistar雌性大鼠。腹腔注入CO(150ml/kg)建立CO中毒DNS模型。存活大鼠随机分为对照组和雌激素组。中毒2~6周后水迷宫试验评估动物的智力状态,苏木精-伊红染色观察脑组织病理改变,TUNEL法检测脑组织细胞凋亡。结果:实验动物腹腔注入CO后出现智力下降,脑组织细胞凋亡明显,符合DNS表现。雌激素干预可显著改善模型动物智力,减轻脑组织病理损害,降低细胞凋亡率。雌激素主要通过抑制凋亡相关信号通道Bcl-2来实现对神经的保护作用。结论:早期雌激素干预可有效降低CO中毒DNS发病率,显著减轻CO中毒DNS损害程度。
Objective: To study the preventive and therapeutic effects of estrogen on the delayed encephalopathy (COI) poisoning of acute carbon monoxide poisoning (CO) and to explore effective clinical treatment. Methods: Adult Wistar female rats were selected. Intraperitoneal injection of CO (150ml / kg) established CO poisoning DNS model. Survival rats were randomly divided into control group and estrogen group. After 2 to 6 weeks of poisoning, the water maze test was used to assess the intelligence state of animals. The pathological changes of brain tissue were observed by hematoxylin-eosin staining and the apoptosis of brain tissue was detected by TUNEL method. Results: The experimental animals with intraperitoneal injection of CO appeared mental decline, brain cell apoptosis significantly, in line with DNS performance. Estrogen intervention can significantly improve the intelligence of model animals, reduce the pathological damage of brain tissue and reduce the rate of apoptosis. Estrogen mainly through the inhibition of apoptosis-related signaling pathway Bcl-2 to achieve the neuroprotective effect. Conclusion: Early estrogen intervention can effectively reduce the incidence of CO poisoning DNS, significantly reduce the damage caused by CO poisoning DNS.